Because ALS typically manifests functional deficits in motor neurons during the later stages of disease progression, we focused on AS events from day 28 iMNs to day 50 iMNs and identified 633, 358, 315, 314, and 260 significant AS events in the FUS, TARDBP, SOD1, C9orf72, and healthy subgroups, respectively (Figures 5A and S4A). This evidence concerns the gene C9orf72 and amyotrophic lateral sclerosis.