We addressed common and specific gene expression changes, alternative splicing (AS) dysregulation, and specific splicing factors regulating AS events throughout motor neuron development and progression in patients with C9orf72-ALS, FUS-ALS, TARDBP-ALS, or SOD1-ALS using temporal RNA sequencing (RNA-seq) profiling. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.