The TCDCA-FXR activation in endothelial cells upregulated ATF4 transcription, which was suppressed by PHB1, thereby enhancing serine and one-carbon metabolism.<h4>Conclusions</h4>This study suggests CDCA as a promising biomarker for identification of obesity-induced ED. This evidence concerns the gene PHB1 and obesity due to melanocortin 4 receptor deficiency.