To address the poorly understood GC N-glycome, we extracted N-glycans from tumor tissue sections via on-site N-deglycosylation using PNGase F. Briefly, N-glycans were directly released from minimal amounts of tumor tissue immobilized on glass slides (≥ 10 μm thick), permethylated, and analyzed by nanoLC-MS/MS. Here, NGLY1 is linked to neoplasm.