Moreover, the impact of the most promising compound was further evaluated in vitro against multi-kinases, B-Raf (V600E), c-Met, Pim-1, EGFR (WT), and VEGFR-2 enzymes, in addition to the cancer cell cycle and the capacity to trigger apoptosis to reveal the anticipated mechanism of anti-proliferative activity. This evidence concerns the gene EGFR and cancer.