By performing dimensionality reduction and clustering on high-throughput sequencing datasets from 12 types of cancer, we categorized the cells within the samples into the following subpopulations: Alveolar, B cells, Cholangiocytes, CD4+ Tconv, CD8+ T cells, CD8+ Tex, Dendritic Cells (DCs), Endothelial, Epithelial, Endometrial stromal cells, Fibroblasts, Hepatocytes, Monocytes/Macrophages (Mono/macro), Mast cells, Myofibroblasts, Microglia, Mural cells, Natural Killer (NK) cells, Neutrophils, Plasma cells, Pericytes, Smooth Muscle Cells (SMC), Trpolif and so on (Figures 4A,C). The gene discussed is CD4; the disease is cancer.