(30) were among the first to identify both EGFR upregulation and mutations in HCC, and demonstrated upregulation in 32.5% of human HCC tissue samples using immunohistochemistry, as well as identifying a number of mutations in exons 19–23 using sanger sequencing and fragment analysis by capillary electrophoresis (30). The gene discussed is EGFR; the disease is hepatocellular carcinoma.