PROM1 and colorectal carcinoma: The cellular thermal shift assay (CETSA) further confirmed the drug-protein interactions (Molina et al, 2013; Jafari et al, 2014; Rai et al, 2017), showing increased thermostability and accumulation of CD133 in CRC cell lines treated with either PP10 or PP24 compared to control groups (Fig. 2I,J; Appendix Fig. S1C,D).