To characterize macrophages in TB lesions and identify pathways associated with the loss of Mtb control, we performed spatial transcriptomics analysis of intralesional macrophages (Iba1+) using the Nanostring GeoMX Digital Spatial Profiler (DSP) system (Merritt et al., 2020; Figure 7—figure supplement 3A-C). The gene discussed is AIF1; the disease is tuberculosis.