Another prominent marker within this subset of proteins was SRS35A (TGME49_280570), also known as bradyzoite-related surface antigen SAG4 that was practically absent in the Type III strains (TgShSp24 and TgShPigSp1) (Supplementary Figure S3), and SRS44 (TGME49_264660), identified as CST1 protein, a well-recognized tissue-cyst marker that showed minor abundance in TgShSp16 and TgPigSp1 isolates (Supplementary Figure S3). Here, CST1 is linked to cyst.