To investigate the mRNA expression of FXR and two of its targets (ABCB11 encoding BSEP and NR0B2 encoding SHP; we have used the protein names in the following for coherence throughout the manuscript) in the PFIC patient carrying the T296I variant, we performed qPCR analysis using FFPE samples from the patient's liver taken at the time of transplantation. This evidence concerns the gene NR1H4 and progressive familial intrahepatic cholestasis.