Compared with children without obesity, children with obesity had 251 promoters and 575 CGIs demethylated, and 141 promoters and 277,251 promoters hypermethylated. Four genes (FZD7, PRLHR, EXOSC4, and EIF6) with differential promoter methylation were validated. This evidence concerns the gene EIF6 and obesity due to melanocortin 4 receptor deficiency.