Total mean fluorescence intensity of vGLUT1 and ELKS1 revealed that within gray matter there was no difference in expression of either vGLUT1 or ELKS1 (Figure 4A and B), but within white matter ELKS1 showed a significant two‐way interaction (Figure 4B and D; interaction, F[1, 34] = 4.182, p = 0.0487), in which AD females showed higher ELKS1 expression compared to control females (Figure 4D; p = 0.0116). This evidence concerns the gene SLC17A7 and Alzheimer disease.