Upon analyzing gene expression data from 457 colon adenocarcinoma (COAD) patients and 166 rectal adenocarcinoma (READ) patients within the TCGA cancer genome database, as well as 4 clinical studies on CRC from the GEO database, it was evident that P2X4 gene expression is notably downregulated in tumor tissues compared to normal tissues (Figure S4A). This evidence concerns the gene P2RX4 and colon adenocarcinoma.