P2RX4 and reading: Upon analyzing gene expression data from 457 colon adenocarcinoma (COAD) patients and 166 rectal adenocarcinoma (READ) patients within the TCGA cancer genome database, as well as 4 clinical studies on CRC from the GEO database, it was evident that P2X4 gene expression is notably downregulated in tumor tissues compared to normal tissues (Figure S4A).