Combination EA35TMZ320 reduced the transcription of WNT5A, an upstream intracellular member (Yu et al., 2007; Chen et al., 2021) known to play a pro-tumor role in glioma (Zuccarini et al., 2018; Chen et al., 2021) to induce the migration of GBM cells (Lee, 2016) to increase cell proliferation (Yu et al., 2007; Chen et al., 2021) and to correlate with higher WHO histological glioma classification grades (Alkailani et al., 2022). This evidence concerns the gene WNT5A and glioblastoma.