Intestinal microbiota is an important factor affecting intestinal immunity, imbalance of microbiota causes pathogen-associated molecular patterns (such as LPS) to enter the blood circulation through the damaged intestinal barrier, activate the Toll-like receptor (TLR4/2) signaling pathway, trigger excessive inflammatory response (such as TNF-α, IL - 6 release), and then cause systemic inflammatory response syndrome (SIRS) and MODS (Adelman et al., 2020; Kullberg et al., 2021). The gene discussed is TNF; the disease is systemic inflammatory response syndrome.