Through next-generation sequencing, single nucleotide polymorphism array, fluorescent in situ hybridization, and polymerase chain reaction-based techniques, the genomic structure and evolutionary mechanisms of seven MYC ecDNA+ tumor cell lines were analyzed, revealing that ecDNA are gradually formed through multiple steps such as amplification, recombination, and deletion of ancestral episomes from a single chromosome (28). Here, MYC is linked to neoplasm.