Though PGE2,19, 20, 21, 22, 23 IL‐8,24, 25, 26, 27, 28 and LPS15, 29, 30, 31, 32 have been used to develop other biosensors for body fluid analysis for various disease models, this work is the first to demonstrate their combinatorial use for multiplexed quantification through label‐free electrochemical biosensing in human urine (with no sample preparation at the user end) for UTI and rUTI diagnosis and management and risk of relapse prediction. This evidence concerns the gene CXCL8 and bacterial urinary tract infection.