In this work, we report a novel proof‐of‐concept biosensing diagnostic tool for the multiplexed electrochemical quantitation of a unique combination of three UTI‐relevant biomarkers, Prostaglandin E2 (PGE2), Interleukin‐8 (IL‐8), and Lipopolysaccharide (LPS), in unfiltered human urine. Here, CXCL8 is linked to bacterial urinary tract infection.