To clarify whether IRF-1 regulates O-GlcNAcylation of NMDARs, IRF-1 was overexpressed in 3xTg-AD brains, or IRF-1 was knocked down in IRF-1 KO mice brains, then western blot was performed to examine the protein levels of IRF-1, GluN1, GluN2A, or GluN2B respectively. This evidence concerns the gene GRIN2B and Alzheimer disease.