IRF1 and Alzheimer disease: To further determine the relationship between IRF-1 and O-GlcNAcylation, we detected O-GlcNAcylation levels in HEK-293T cell lysate overexpressing IRF-1 (Fig. 2D-E), as well as in homogenates from the brain of IRF-1 knockout mice (Fig. 2F-G) and 3xTg-AD mice injected with AAV-IRF-1 (Fig. 2H-I).