For virus flow cytometry, 5 × 108 washed platelets/mL were incubated with SFV nsP3-eGFP (multiplicity of infection (MOI) = 10) or an equivalent volume of low serum EMEM (mock) for 3.5 h followed by three centrifugal washes at 800g for 10 min. Here, SH2D3C is linked to infection.