Indeed, in the chemical itch model, blocking the NPY-Y1R-signaling pathway does not diminish the itch inhibition mediated by VGLUT3+ sensory fibers (histamine: t = 0.3059, p = 0.7641, two-tailed unpaired t test; chloroquine: t = 0.02711, p = 0.9787, two-tailed unpaired t test) (Fig. 5B, C). This evidence concerns the gene NPY and Pruritus.