Further assessment of senescence markers on SSc cells treated with 10 μM BMS-470539 demonstrates increased in SA-βGal and lipofuscin accumulation (Fig. 2A), maintenance of metabolic activity (Fig. 2B), increase in cell size (Fig. 2C), upregulation of cyclin-dependent kinase inhibitors CDKN1A and CDKN2A (Fig. 2D), presence of binucleated cells (Fig. 2E) and reduced cell proliferation (Fig. 2F). Here, CDKN1A is linked to systemic sclerosis.