We next compared the differential gene expression between KPCA.FSTKO and KPCA.FSTKO_hFSTL3 stromal cluster and observed a significantly increased expression (Log2 FC>0.5) of myCAF markers (Acta2, Tagln), collagen (Col8a1), and secreted factor (Pdgfa) consistent with a myofibroblast phenotype in the KPCA.FSTKO_hFSTL3 tumor. This evidence concerns the gene ACTA2 and neoplasm.