For comparison, another HSP54-associated mutant p.Asp660His41 (DDHD2D660H-myc), and a catalytically inactive p.Ser351Ala mutant1,12 (DDHD2S351A-myc), were also introduced into Ddhd2−/− neuron–glia cultures at DIV 3 using a multiplicity of infection (MOI) of 1. Here, MYC is linked to infection.