HSF1 and cancer: To investigate the nature of HSF1- and DBC1-bound sites and SE dynamics during cancer progression, we profiled active chromatin regions using H3K27ac ChIP-seq (Supplementary Fig. 8a) and found a massive remodeling of active chromatin regions, with 22,975 upregulated and 6,542 downregulated H3K27ac regions, in SM1 compared with 22RV1 cells (Fig. 5h and Supplementary Fig. 8b).