To investigate the nature of HSF1- and DBC1-bound sites and SE dynamics during cancer progression, we profiled active chromatin regions using H3K27ac ChIP-seq (Supplementary Fig. 8a) and found a massive remodeling of active chromatin regions, with 22,975 upregulated and 6,542 downregulated H3K27ac regions, in SM1 compared with 22RV1 cells (Fig. 5h and Supplementary Fig. 8b). The gene discussed is CCAR2; the disease is cancer.