PSPC1 and renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions: To further validate the role of PSPC1 in supporting tRCC cell growth, we depleted PSPC1 using shRNA-mediated knockdown (Supplementary Fig. 5f) followed by a colony formation assay in both UOK109 (with NONO-TFE3 fusion) and 786-O (without fusion) cells.