The cGAS-STING is essential in mediating the inflammatory response,33,34 and its activation is linked to various cardiovascular disorders.35–37 Notably, a recent study indicated that STING knockout alleviates diabetes-associated VC in mouse models.38 Moreover, the heatmap demonstrated multiple genes within the cGAS‒STING pathway and downstream inflammatory factors was significantly decreased after LINE1 inhibition by si-LINE1 or NRTIs compared with that in the controls (Fig. 5h, i). The gene discussed is CGAS; the disease is cardiovascular disorder.