ZAP70 and neoplasm: Further investigation revealed that the TCR signaling pathway was significantly activated in hCT3 AbTCR T cells after co-culturing with NBEB tumor cells for 2 h compared to mock, CT3 CAR, and CT3 AbTCR T cells, with increased expression of phosphorylated ZAP70, SLP76, and LAT (Figures 6E and 6F).