This improvement in GVHD severity with ACC1KO Tregs was correlated with reduced donor Tcon proliferation early (day 3) after transplant (Figure 3D), and reduced inflammatory cytokine production by donor CD4+ and CD8+ Tcons on day 14 (D14) after transplantation (Figure 3, E–H). This evidence concerns the gene CD8A and graft versus host disease.