Treating fourth-instar or female adult BPH with the LC50 concentration of EB significantly increased the expression of AT, ATR, and AstCCC, while resulting in the downregulation of AstA, AstB/mip, AstCC, AstAR, and AstBR/mipr at the adult stage (Figure 4A and B, Figure 4—figure supplement 3). Here, ATR is linked to benign prostatic hyperplasia.