CALCA and infection: We first assessed whether antistaphylococcal agents representing different pharmacologic classes alter KC immunometabolism at baseline (without infection) by performing RT-qPCR and FLIM following 10 h exposure to vancomycin (VAN), ceftobiprole (CTB), daptomycin (DAP), and tedizolid (TED) at reported human Cmax concentrations (Fig. 3A through C).