Functionally, blocking either LFA-1 or DNAM-1 significantly reduced TPNC-mediated cytotoxicity against multiple MM cell lines (IM-9, RPMI8226, U266) as well as the autologous RRMM-#10 sample (Fig. 3F–I), confirming that these non–MHC-restricted pathways contribute substantially to TPNC cytotoxicity. This evidence concerns the gene CD226 and Miyoshi myopathy.