These findings indicate that Tenuigenin inhibits LPS-induced injury and dysfunction of HPMECs by inhibiting SRC pathway activity, revealing that the SRC pathway serves as a potential new target for intervening in LPS-induced pulmonary endothelial injury for the first time, laying a crucial theoretical foundation for developing more precise treatment strategies for adult pneumonia based on Tenuigenin or its derivatives. The gene discussed is SRC; the disease is pneumonia.