It has been demonstrated that non-autologousosomes have the capacity to encapsulate CRISPR/Cas9 plasmid DNA using common transfection reagents to target the mutant KRAS G12D oncogene in PC.This approach has been established to reduce cancer cell proliferation and inhibit the growth of PC cells in both subcutaneous and orthotopic models. Here, KRAS is linked to pachyonychia congenita.