To understand how Rab7a affects the disassembly of BBB tight and adherens junctions during the acute phase of ischemic stroke, we analyzed the morphology of eGFP::Claudin-5+, ZO-1+ TJs and CDH5+ AJs at 48 hours after t-MCAO by immunofluorescence and electron microscopy. This evidence concerns the gene RAB7A and ischemic stroke.