To determine the effects of endothelial Rab7a elimination on AJ (VE-Cadherin) and TJ [Claudin-5, Occludin, and ZO-1] protein levels in BECs after ischemic stroke, lysates were collected from either the ipsilateral (core region) and contralateral cortices of both genotypes at 48 h after t-MCAO and assessed by Western blotting (Fig. 6D). The gene discussed is CLDN5; the disease is ischemic stroke.