We induced ischemic stroke in Rab7afl/fl and Rab7aiECKO mice by performing t-MCAO for 45 min [11] and analyzed the effect of Rab7a EC-specific elimination on acute changes in paracellular BBB permeability at 48 h after ischemic stroke following injection of a small dye biocytin-tetramethyl rhodamine (biocytin-TMR; 890 Da) that preferentially extravasates from blood vessels through the paracellular route [11, 12]. This evidence concerns the gene RAB7A and ischemic stroke.