A comprehensive analysis of genetic associations between somatic mutations and iNMDeff across TCGA tumors, analyzing over 6.4 million tests across various cancer and NMD-related genes (see List in Additional File 2: Table S9) and cancer types, revealed only two significant associations: a missense variant in the TLX1 gene in lung adenocarcinoma (LUAD; linked with enhanced iNMDeff) and a truncating variant in the CDH1 gene in breast invasive carcinoma (BRCA; linked with decreasing iNMDeff). This evidence concerns the gene TLX1 and invasive breast carcinoma.