In conclusion, the pathological manifestations of antibody-negative PBC and antibody-positive PBC in this study were similar, but the lymphocyte aggregation was more severe, while the bile duct injury and CK7 positive hepatocytes were less severe in antibody-negative PBC than in antibody-positive PBC, suggesting that the overall pathological changes of antibody-negative PBC were slightly milder than those of antibody-positive PBC. The gene discussed is KRT7; the disease is primary biliary cholangitis.