To examine the role of MARCH8 in PC metastasis in vivo, we carried out zebrafish xenograft experiments by injecting stable CFPAC-1 cells into the peri-vitelline space (PVS) of 2-day post-fertilization (dpf) zebrafish (Fig. 9H) and monitored metastatic events four hours post-injection (Martinez-Lopez et al, 2021; White and Patton, 2023). Here, MARCHF8 is linked to pachyonychia congenita.