Next, to further investigate the phenotype of CD8+ T cells in Ri-AIE, we performed single-cell RNA sequencing (scRNAseq) on sorted ex vivo circulating CD8+ T cells from seven patients with Ri-AIE (including Ri01 patient for whom we had found the neuron-reactive CD8+ T cell clonotype) as well as three sex- and age-matched donors without an autoimmune disease (AgD) (Fig. 4a, Supplementary Tables 4 and 5). Here, CD8A is linked to autoimmune disease.