KCNJ11 and Hypoglycemia: COSm6 cells were cotransfected with human WT or mutant SUR1 and WT Kir6.2 complementary DNAs (cDNAs), preloaded with Rb+, and subjected to Rb+ efflux measurement over a 30-min period in Ringer's solution that contained metabolic inhibitors (2.5 μg/ml oligomycin and 1 mM 2-deoxy-D-glucose; referred to as MIs hereinafter) which decrease intracellular ATP/ADP ratios to activate KATP channels, mimicking the effect of hypoglycemia (see Experimental procedures).