Moreover, endothelial insulin resistance may have contributed to the whole‐body glucose metabolism abnormalities observed in VeNKO mice, as endothelial eNOS phosphorylation at Ser‐1177 is regulated by Akt signaling (Dimmeler et al. 1999) and Sirt3 deficiency in vascular endothelial cells induces endothelial insulin resistance (Yang et al. 2016). This evidence concerns the gene NOS3 and Insulin resistance.