LCN2 and acute kidney injury: In pediatrics, the best evidence in support of this practice has been demonstrated in the ICU, where the Trial in AKI using NGAL and Fluid Overload to optimize CRRT use (TAKING FOCUS 2; TF2) demonstrated that a sequential risk stratification process including the Renal Angina Index (RAI) calculated 12 h after pediatric ICU admission followed by urine neutrophil gelatinase-associated lipocalin (uNGAL) measurement in patients identified as high risk both accurately predicted severe AKI and improved patient outcomes [11, 12].