In fact, a significant increase in the percentages and absolute numbers of TAMs, identified as CD11b+F4/80+ cells, as well as M1-like macrophages, identified as CD11b+F4/80+CD86+ cells, were observed in the TME of tumor-bearing mice inoculated with SEMA7A-knockdown LLC cells (Figures 3B, C), but the percentages and absolute numbers of M2-like macrophages, identified as CD11b+F4/80+CD206+ cells were significantly decreased (Figure 3C), resulting in an increase in the M1/M2 ratio (Figure 3D). This evidence concerns the gene CD86 and neoplasm.