Immunohistochemistry (IHC) analysis revealed significantly upregulated expressions of SP1, TGF‐β, p‐Smad2, and p‐Smad3 in patients with CRC and liver metastasis compared with those with CRC without liver metastasis (Figure8A; Figure S15A, Supporting Information). This evidence concerns the gene TGFB1 and colorectal carcinoma.