To confirm whether VIRMA can be lactylated at K1713 in cells, we transiently transfected FLAG‐tagged wild‐type VIRMA (FLAG‐VIRMA) or a lactylation‐deficient mutant (FLAG‐VIRMA(K1713R)) into CRC cells and treated them with 25 mm lactate. Here, VIRMA is linked to colorectal carcinoma.