TSC2 and tuberous sclerosis: To explore dysregulated gene expression in TSC, mRNA sequencing (RNAseq) data from 15 TSC patient SEN/SEGA samples were compared to non-TSC brain tissue (as previously described [26]), and RNAseq data from TSC2(−) AML cells (621 − 101) were compared to those from TSC2(+) AML cells (621 − 103).