Nearly all (~ 90–93%) p62 inclusions were LAMP1 + in MAPT mice at both ages and 4-month AppNL−G−FxMAPT mice, yet a significant loss (P < 0.0001) of co-localization was observed in the 12-month AppNL−G−FxMAPT mice (~ 57% co-localization; Fig. 6K,L), indicating a mounting impediment in autophagic flux in sleep-regulating neurons in the AD mice. This evidence concerns the gene LAMP1 and Alzheimer disease.