We employed a unilateral destabilization of the medial meniscus (DMM) to establish a murine knee osteoarthritis (OA) model (Fig. 2F, G) and, through qPCR analysis, observed a significant decrease in IRS2 mRNA levels in the articular cartilage of DMM-induced OA knee joints (Fig. 2H). This evidence concerns the gene IRS2 and osteoarthritis, knee.