Mice inoculated with 4T1 mammary carcinoma cells had enhanced IFN-γ secretion and MUC1-specific CD8+ T cell killing when immunized with 10 μg of a MUC1 mRNA nanovaccine encapsulated in lipid/calcium/phosphate nanoparticles (LCP) compared to mRNA or LCP alone. This evidence concerns the gene MUC1 and breast carcinoma.