Mechanistic studies showed that Compound 8 (Figure 5) was able to block the cell cycle at the G0/G1 phase by up-regulating the expression of the cycle regulatory proteins p21cip1/waf1 and p27kip1, as well as down-regulating the expression of cyclin E and cyclin D3, and promote apoptosis by inhibiting the expression of Bcl2 and promoting the expression of Bax proteins, to achieve an anti-tumor effect [112]. This evidence concerns the gene CDKN1A and neoplasm.