IRF3 and Flavivirus Infections: Additional experiments were performed in the context of flavivirus infection with similar outcomes: both MAVS and IRF3 failed to colocalize with G3BP1 in the context of WNV and ZIKV infection, and G3BP1 knockout failed to alter IRF3 phosphorylation in WNV-infected cells or nuclear translocation in WNV and ZIKV-infected cells.