Macrophage speeds for our 2D/3D infection model (7.61 × 10−4 μm/s, 3.8 × 10−4 μm/s) and 3D control (2.75 × 10−4 μm/s) were comparable to macrophage speeds observed for CSF-1-starved cells (~6.39 × 10−4 μm/s), which also exhibited a more rounded morphology similar to our 3D and infected 2D cultures. This evidence concerns the gene CSF1 and infection.